Insert materials into the tank to convert them into fuel. You can freely call upon this item, but summoning it will not work in certain areas.
Using the Master Cycle 0
The Motorcycle does require "fuel" to function. Link can hold items from his inventory and then go next to the motorcycle and "drop" them in. The fuel gauge will be displayed, showing how much it filled up the tank of the motorcycle.
Link can still fight on the motorcycle, but you must remember to hold down the accelerator or Link will slow down.